Xenograft tumor model

DISEASE AREA Oncology THERAPEUTIC AREAS Various tumor model

Xenograft Model

The Xenograft model is a model in which human-derived cancer cells are transplanted into immunodeficient mice. The subcutaneous xenograft tumor model is the most common model for determining the in vivo activity of anticancer therapeutics. The anti-tumor effect of the test substance is evaluated by measuring and calculating the tumor volume in tumor-bearing mice.
The advantages of the human tumor xenograft model are 1) it predicts the drug response of tumors in human patients, 2) it can be administered locally to tumors, and 3) it allows rapid evaluation of human tumor response in a short period of time.
In addition to drug efficacy evaluation studies, this model is also used to obtain cancer cell-derived samples.

Cell lines used for model generation can be purchased from any cell bank (ATCC, etc.).
Below are examples of cell lines that can be used.

  • PANC-1 (human pancreatic cancer)
  • HTC-116 (human colon cancer)
  • Caco-2 (human colon cancer)
  • Hep G2 (human liver cancer)
  • Hela (human cervical cancer)
  • A549 (human lung cancer)

Fig. Subcutaneous xenograft tumor model using HeG2. The tumor is marked by a red circle.